关键词: 木绣球花, 绿原酸, 索氏提取, 高效液相色谱

Abstract: The contents of chlorogenic acid in flowers,torus,stems and leaves of Viburnum macrocephalum was determined by HPLC. V.macrocephalum was extracted by Soxhlet extraction method.The chromatographic condition was established as follows:Eclipse XDB-C₁₈ (4.6 mm × 250 mm,5 μm) column,the mobile phase composed of acetonitrile-0.1% phosphoric acid aqueous solution (10:90),the detection wavelength was 327 nm,the flow rate was 1.0 mL/min.Under the developed HPLC conditions,good linear relationship was established within the range of 0.346.80 μg/mL.The content of chlorogenic acid in different parts of V.macrocephalum was in following order:torus (1.14%)>flowers (1.02%)>stems (0.51%)>leaves (0.45%).The torus of V.macrocephalum had the highest content of chlorogenic acid. V macrocephalum had the potential exploitation value.

Key words: Viburnum macrocephalum, chlorogenic acid, soxhlet extractor, HPLC