关键词: 枇杷, 细胞悬浮培养, 熊果酸, 超声提取, 高效液相色谱

Abstract: Single factor,orthogonal experiments and variance analysis were adopted to determine the process of the cells collection,extraction and content assaying of UA from loquat suspension cell cultures.The results showed that:the appropriate collection method was filtering through four-layer gauze using Buchner funnel. Ultrasonic extraction conditions were:95% ethanol as extraction solvent,ultrasonic power 50 W,ultrasonic time 25 min,solid-liquid ratio 1:40,ultrasonic 2 times.HPLC chromatographic conditions:mobile phase consisted of methanol and water -acetic acid-triethylamine = 10:0.03:0.06 (volume ratio),with volume ratio of 83:17;flow rate 1.0 mL/min;detection wavelength was 203 nm;column temperature was 30 ℃.The average recovery rate for the determination of ursolic acid was up to 99.3%.

Key words: Eribotrya japonica, cell suspension, ursolic acid, ultrasonic extraction, HPLC