天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl2): 313-316.

• 开发研究 • 上一篇    下一篇

红花花冠总RNA提取方法的筛选研究

康亚兰1,2,裴瑾1*,刘薇1,罗静1,刘维1,陈翠平1,吴清华1   

  1. 1 成都中医药大学药学院,中药资源系统研究与开发利用国家重点实验室培育基地,成都 610075;2 成都医学院药学院,成都 610083
  • 出版日期:2014-12-30 发布日期:2015-03-05

The Screening Study of Method of Total RNA Extraction of Safflower Corolla

KANG Ya-lan1,2,PEI Jin1*,LIU Wei1,LUO Jing1,LIU Wei1,CHEN Cui-ping1,Wu Qing-hua1   

  1. 1 School of Pharmacy,Chengdu University of Traditional Chinese Medicine,State Key Laboratory Breeding Base of the Research and Development for Traditional Chinese Medicine Resources,Chengdu 610075,China; 2 Phamaceutical College,Chengdu Medical College,Chengdu 610083,China
  • Online:2014-12-30 Published:2015-03-05

摘要: 筛选一种红花花冠总RNA提取方法。采用植物总RNA提取试剂盒法、传统Trizol法、改良Trizol法提取红花花冠总RNA,凝胶电泳、RT-PCR、紫外分光光度法检测三种方法提取所得总RNA,并将筛选得到的方法应用于红花愈伤组织总RNA的提取。改良Trizol法提取得到的红花花冠总RNA凝胶电泳检测可见清晰的三条带,RT-PCR结果凝胶电泳检测可见清晰的一条带,紫外分光光度法检测符合要求,其余两种方法均不符合要求。改良Trizol法应用于红花愈伤组织总RNA提取,检测结果均符合要求。改良Trizol法可用于红花花冠总RNA提取,提取所得红花花冠总RNA可用于后续分子生物学实验。

关键词: 红花花冠, 总RNA提取, 植物总RNA提取试剂盒, 传统Trizol法, 改良Trizol法

Abstract: A method used for the extraction of safflower corolla was screened.Plant total RNA extraction kit,traditional Trizol,improved Trizol were used for the extraction of safflower corolla.Gel electrophoresis,RT-PCR and ultraviolet spectroscopy were used for detection.And the method screened was used for the total RNA extraction of safflower callus.The total RNA extraction of safflower Corolla used improved Trizol obtainedclearly visible gel electrophoresis three bands,RT-PCR results visible gel electrophoresis with a clear,UV spectrophotometry meet the requirements,the other two methods do not meet the requirements.The improved Trizol can used for the extraction of safflower corolla,the obtained total RNA can be used for the following experiment of Molecular Biology.

Key words: safflower corolla, total RNA extraction, plant total RNA extraction kit, traditional Trizol, improved Trizol

中图分类号: 

R284.2