天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (4): 655-660.doi: 10.16333/j.1001-6880.2015.04.020

• 研究简报 • 上一篇    下一篇

沙棘枝叶与浆果黄酮抗氧化活性比较

焦扬1,李彩霞1,高海宁1,冯九海2*   

  1. 1 河西学院农业与生物技术学院;2 甘肃省应用真菌工程实验室,张掖 734000
  • 出版日期:2015-04-28 发布日期:2015-05-08

Antioxidant Activity of Flavonoids from Branches Leaves and Berry of Hippophae rhamnoides Linn

JIAO Yang1,LI Cai-xia1,GAO Hai-ning1,FENG Jiu-hai2*   

  1. 1 College of Agriculture and Biotechnology,Hexi University;2 Gansu Engineering Laboratory of Applied Mycology,Gansu Zhangye 734000,China
  • Online:2015-04-28 Published:2015-05-08

摘要: 以大果沙棘浆果黄酮(ZYCFJ)为参照,考察了张掖大果沙棘黄酮(ZYCF)和新疆野生沙棘枝叶黄酮(XJCF)的抗氧化活性。结果表明:沙棘浆果黄酮和沙棘枝叶黄酮对不同自由基的清除效果均有差异。在黄酮浓度为50 μg/mL时, ZYCFJ、ZYCF和XJCF对ABTS+清除率分别为60.8%、42.6%、45.4%;对DPPH的清除率分别为95.7%、80.6%、88.6%,在黄酮浓度高于40 μg/mL时,ZYCFJ对DPPH和ABTS+的清除效果比ZYCF和XJCF的好。在黄酮浓度为500 μg/mL时,ZYCFJ、ZYCF和XJCF对·OH的清除率分别为92.7%、88.9%、98.6%;对O2的清除率分别为 76.5%、70.6%、82.4%;对亚硝酸盐的清除率分别为98.7%、76.8%、83.7%。就对亚硝酸盐的抑制和Fe3+的还原来说,ZYCFJ明显优于ZYCF和XJCF;对超氧阴离子和羟自由基的清除效果ZYCFJ、ZYCF和XJCF的相当。

关键词: 沙棘枝叶, 黄酮, 张掖大果沙棘, 新疆野生沙棘, 抗氧化性

Abstract: Using flavonoids from Hippophae rhamnoides L.berry (ZYCFJ) as reference,the antioxidant activities of flavonoids in branches and leaves of Hippophae rhamnoides L.from Zhangye (ZYCF) and Xinjiang (XJCF) were evaluated by measuring their scavenging activity against DPPH,ABTS,hydroxyl,superoxide anion radicals and reducing Fe3+ and inhibiting nitroso.The results showed that at 50 μg/mL concentration level,the clearance rates of ZYCFJ,ZYCF and XJCF on ABTS+ were 60.8%,42.6%,45.4%,and on DPPH were 95.7%,80.6%.88.6%.When flavonoids concentrations higher than 40 μg/mL,the scavenging activity against DPPH and ABTS+ of ZYCFJ was better than XJCF and ZYCF.At 500 μg/mL concentration level,the scavenging rates of ZYCFJ,ZYCF and XJCF on ·OH were 92.7%, 88.9%, 98.6%,and on O2- were 76.5%,70.6%,82.4%,and inhibition ratio of ZYCFJ,ZYCF and XJCF on nitrite were 98.7%,76.8%,83.7%.In term of the inhibition rates of nitrite and the reduction of Fe3+,ZYCFJ was better than that of ZYCF and XJCF.The scavenging effect of ZYCFJ,ZYCF and XJCF on superoxide anion and hydroxyl free radical were equivalent.

Key words: Hippophae rhamnoides L.branch;flavonoids, Hippophae rhamnoides L.from Zhangye, Hippophae rhamnoides L.from Xinjiang;antioxidant activity

中图分类号: 

R285