天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (4): 687-690.doi: 10.16333/j.1001-6880.2015.04.025

• 开发研究 • 上一篇    下一篇

秀丽线虫天然粗提物对哮喘治疗效果研究

黄月娥1,2,李朝品1,3*,姜玉新3,郭伟3,黄小梅3   

  1. 1 安徽理工大学地球与环境学院,淮南232001;2 皖南医学院公共卫生学院;3 皖南医学院基础医学院,芜湖241002
  • 出版日期:2015-04-28 发布日期:2015-05-08

Treatment Effect of Crude Extract of Caenorhabditis elegans on Allergic Asthma

HUANG Yue-e 1,2, LI Chao-pin1,3*,JIANG Yu-xin3,GUO Wei 3,HUANG Xiao-mei3   

  1. 1 School of earth and environment,Anhui University of Science and Technology,Huainan 232002,China; 2 School of Public Health ,Wannan Medical College; 3 School of Basic Medicine,Wannan Medical College,Wuhu 241002,China
  • Online:2015-04-28 Published:2015-05-08

摘要: 探讨秀丽隐杆线虫天然粗提物的提取及对哮喘动物治疗效果。将线虫经离心、洗涤、超声乳化、去除内毒素等方法获得线虫粗提物(CEC),以鸡卵清蛋白(OVA)致敏BALB/c雌性小鼠建立哮喘动物模型,用CEC与OVA混合物(CE)对哮喘动物治疗,以缓冲液(PBS)和OVA为对照,用ELISA法检测血清IgE、IgG1、IgG2a抗体,分类计数支气管肺泡灌洗液(BALF)细胞,切取肺组织观察病理。研究显示,CE组与OVA组比较,血清IgE、IgG1、IgG2a差异有统计学意义(P<0.05),BALF中细胞总数、嗜酸性粒细胞、巨噬细胞等差异有统计学意义(P<0.05)。线虫天然粗提物对哮喘动物有一定治疗效果。

关键词: 秀丽隐杆线虫, 粗提物, 哮喘, 治疗效果

Abstract: To investigate the extraction of Caenorhabditis elegans and its effect on curing animals with allergic asthma. The crude extract of C. elegans (CEC) was obtained through centrifugation,washing,phacoemulsification and endotoxin removal. Sensitizing BALB/c mice with ovalbumin (OVA) was used to develop the animal model of asthma. Animals with asthma were treated with a mixture of CEC and OVA (CE). Buffer solution (PBS) group and OVA group were set as control groups. The serum levels of IgE,IgG1 and IgG2a were measured with commercial ELISA kits,and the quantity of cells in the bronchoalveolar lavage fluid (BALF) were counted. Lung tissues were removed to observe its histopathology. The results showed that the differences in the serum levels of IgE,IgG1 and IgG2a of CE group and OVA group were statistically significant (P<0.05). The differences in the quantity of total cells,eosinophils and macrophages in BALF were statistically significant (P<0.05). It showed that CEC have therapeutic effect on animals with allergic asthma.

Key words: Caenorhabditis elegans, crude extract, allergic asthma, treatment effect

中图分类号: 

R186