天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (4): 691-694.doi: 10.16333/j.1001-6880.2015.04.026

• 开发研究 • 上一篇    下一篇

云南昭通年青褐煤黄腐酸钠对血管新生作用的影响

杨静,何静*,丁亚芳,庄文婷,向诚,秦谊,李宝才   

  1. 昆明理工大学 生命科学与技术学院,昆明 650500
  • 出版日期:2015-04-28 发布日期:2015-05-08

Angiogenesis Effect of Sodium Fulvate Extracted from Young Lignite of Zhaotong Region in Yunnan Province

YANG Jing,HE Jing*,DING Ya-fang,ZHUANG Wen-ting,XIANG Chen,QIN Yi,LI Bao-cai   

  1. Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China
  • Online:2015-04-28 Published:2015-05-08

摘要: 研究云南昭通地区年青褐煤黄腐酸钠对体内外血管新生作用的影响。将不同浓度的黄腐酸钠作用于人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)体外模型和鸡胚绒毛尿囊膜(chick chorioallantoic membrane,CAM)体内模型,采用MTT和划痕法检测其对HUVECs增殖和迁移的影响,以ELISA试剂盒测定细胞上清液血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达,采集CAM图片用Image-Pro Plus6.0软件考察给药区域血管新生的情况。结果显示,与对照组比较,云南昭通年青褐煤黄腐酸钠在100、200、400 μg/mL的浓度下对HUVECs的增殖有抑制作用(P<0.05,P<0.01),在100、200 μg/mL浓度时对迁移有抑制作用并能下调血管内皮生长因子VEGF的表达(P<0.01),在高浓度800、1200 μg/mL下能抑制CAM血管的新生(P<0.05)。研究结果表明,云南昭通地区年青褐煤黄腐酸钠具有良好的血管新生抑制作用,其机制可能与生长因子VEGF表达的下调有关。

关键词: 黄腐酸钠, 血管新生, 人脐静脉内皮细胞, 鸡胚绒毛尿囊膜, VEGF

Abstract: The purpose of the present study was to evaluate the effect of sodium fulvate on angiogenesis in vivo and in vitro. Sodium fulvate was extracted from young lignite of Zhaotong region in Yunnan province.Human umbilical vein endothelial cells (HUVECs) and chick chorioallantoic membrane (CAM) were treated with different concentrations of sodium fulvate.MTT and scratch assays were applied to analyze HUVECs viability and migration.The expression of autocrine vascular endothelial growth factor (VEGF) in HUVECs was detected by ELISA method.ImagePro Plus6.0 imaging software was used to quantify the area of the photoed blood vessels in CAM.Compared with the untreated group,sodium fulvate with the concentrations of 100,200 and 400 μg/mL can significantly inhibit proliferation of HUVECs (P<0.05, P<0.01).At the concentrations of 100 and 200 μg/mL,the migration of HUVECs was also inhibited and the expression of autocrine VEGF was downregulated as well (P<0.01).CAM assay results showed that the capillary vessels sprouts can be inhibited at the concentrations of 800 and 1200 μg/mL(P<0.05).In conclusion, sodium fulvate extracted from young lignite of Zhaotong region in Yunnan province showed anti-angiogenesis activity,which may be related to the down-regulation of VEGF expression.

Key words: sodium fulvate, angiogenesis, HUVECs, CAM, VEGF

中图分类号: 

R285