天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (4): 732-736.doi: 10.16333/j.1001-6880.2015.04.034

• 开发研究 • 上一篇    下一篇

响应面法优化江永香柚皮多糖的提取工艺

刘小文1,2,敖艳1,2,全沁果1,2,徐思敏1,2,王宗成1,2,齐成媚1,2,何福林1,2*   

  1. 1 湖南科技学院湘南优势植物资源综合利用湖南省重点实验室;2 湖南科技学院生命科学与化学工程系,永州 425199
  • 出版日期:2015-04-28 发布日期:2015-05-08

Optimization of Extraction Process of Polysaccharides from Peels of Citrus Grandis by Response Surface Methodology

LIU Xiao-wen1,2, AO Yan1,2, QUAN Qin-guo1,2, XU Si-min1,2,WANG Zong-cheng1,2,QI Cheng-mei1,2, HE Fu-lin1,2*   

  1. 1 Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South,Hunan University of Science  and Engineering;2 Department of Biochemistry,Hunan University of Science and Engineering,Hunan Yongzhou 425199,China
  • Online:2015-04-28 Published:2015-05-08

摘要: 以我国具有地域特色的江永五香之“香柚”作为原料,采用超声波辅助热水浸提法提取香柚皮多糖。在单因素试验基础上选取影响提取率的主要因素:料液比、超声波处理时间、水浴温度、水浴时间。通过响应面设计,对柚皮多糖的提取条件进行优化设计。结果表明,在提取料液比(m/V)1:25,超声波处理时间(t)3.8 min,水浴温度为(T)70 ℃,水浴时间(t)2.6 h的条件下,提取效果最佳。此条件下柚皮中水溶性多糖平均提取率为5.629%。超声波辅助与传统热水醇沉法比较,耗能较少,提取效率较高,可作为江永香柚皮多糖提取的一种有效方法。

关键词: 江永香柚, 多糖, 提取, 响应面

Abstract: In this study,Jiangyong Citrus grandis (grapefruit),one of the regional characteristics of Jiangyong Wuxiang,were used as raw material for the extraction of polysaccharides. Polysaccharides in peels of C.grandis was extracted with the ultrasonic-assisted hot water extraction method.The influencing factors,including solid/liquid ratio,ultrasonic treatment time,extraction temperature and time,were optimized through response surface analysis tests on the basis of single factor experiment.The results showed that the optimal extraction conditions were solid/liquid ratio of 1:25,ultrasonic treatment time of 3.8 min,extraction temperature of 70 ℃ and extracting for 2.6 h.Under the optimal conditions,the average extraction yield of polysaccharides was about 5.629%.Compared with the conventional hot water and alcohol precipitation method,the ultrasonic assisted extraction used less energy and had higher extraction efficiency.Hence,it was concluded that the ultrasonic assisted extraction method can be used as an effective method for the extraction of polysaccharide from peels of C.grandis.

Key words: Citrus grandis, polysaccharides, extraction, response surface methodology

中图分类号: 

R284.2