关键词: 天花粉, 总黄酮, 正交试验, 工艺研究

Abstract: In this study,the extraction technology of total flavonoids from Radix Trichosanthis was optimized,and the content of total flavonoids was measured using Al(NO₃)₃-NaNO₂ colorimetric method.Based on the single factor experiment,the effect of the volume fraction of methanol,solid-liquid ratio,extraction exmperature,extraction time on the extraction yield of total flavonoids were studied using L₉(3⁴) orthogonal experiment.The results showed that the optimal extraction parameters were as followed:volume fraction of methanol was 50%,ratio of solid to liquid was 1:15,extraction temperature was 70 ^oC,and extraction time was 90 min.The total content of flavone was 0.1184 mg/g under the optimal extraction conditions.The developed method was simple,stable and high repeatable.The content of total flavonoids in Radix Trichosanthis was determined in this study for the first time,which provided the basis for the quality control of Radix Trichosanthis.

Key words: Radix Trichosanthis, total flavonoids, orthogonal test, technical study