天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (7): 1210-1214.doi: 10.16333/j.1001-6880.2015.07.016

所属专题： No.8

• 研究简报 • 上一篇    下一篇

UV和HPLC法测定黄芩黄酮总苷元提取物中总黄酮和3种主要成分的含量

彭易兰1,2，刘云华2，黄志芳2，刘玉红2，陈燕2，易进海2*   

  1. 1 成都中医药大学，成都 611137；2 四川省中医药科学院，成都 610041
  • 出版日期:2015-07-20 发布日期:2015-07-23

Determination of Total Flavonoids and Three Main Components in Scutellariae Radix Total Flavone Aglycone Extract by Ultraviolet Spectrophotometry and Highperformance Liquid Chromatogaphy

PENG Yi-lan1,2, LIU Yun-hua2, HUANG Zhi-fang2, LIU Yu-hong2, CHEN Yan2, YI Jin-hai2*   

  1. 1 Pharmaceutical College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; 2 Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China
  • Online:2015-07-20 Published:2015-07-23

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摘要： 本文建立了黄芩黄酮总苷元提取物中总黄酮和3种主要成分的含量测定方法。采用HPLC法测定黄芩素、汉黄芩素和千层纸素A的含量。使用Agilent 1200型高效液相色谱仪，Eclipse XDB-C18（250 mm×4.6 mm，5 μm）色谱柱，流动相为甲醇-0.2%磷酸水（50:50），流速1 mL / min，柱温35 ℃，检测波长275 nm的方法。黄芩素、汉黄芩素和千层纸素 A线性范围分别为3.501~70.016 μg/mL（r=0.9999），0.832~16.640 μg/mL（r=0.9998），0.418~8.352 μg/mL（r=0.9999）；平均加样回收率（n=6）分别为99.55%、98.86%、9.997%，RSD分别为1.74%、1.74%、1.70%。采用UV法测定总黄酮含量，黄芩素线性范围为1.71~8.54 μg/mL（r=0.9991）；平均加样回收率（n=6）为100.93%，RSD为2.63%。黄芩素、汉黄芩素、千层纸素 A和总黄酮的含量分别为48.22%~60.36%、12.09%~14.46%、5.05%~8.81%、78.11%~90.12%；研究结果表明该方法准确、可靠，适用于黄芩黄酮总苷元提取物的含量测定。

关键词: 黄芩黄酮总苷元提取物, 黄芩素, 汉黄芩素, 千层纸素A, 高效液相色谱法, 紫外分光光度法, 含量测定

Abstract: The paper established a method for separation and determination of total flavonoids and three main components in Scutellaria Radix total flavone aglycone extract by ultraviolet (UV) spectrophotometry and high-performance liquid chromatography.The anlysis was performed on an Agilent 1200 Series HPLC system with Eclipse XDB-C18 (250 mm×4.6 mm,5 μm) column.The mobile phase consisted of methanol-0.2% phosphoric acid water (50:50) with the flow rate of 1 min/mL.The column remained at 35 ℃ and the detection wavelength was set at 275 nm.The linear ranges of baicalein,wogonin and oroxylin A were 3.501-70.016 μg/mL (r=0.9999), 0.832-16.640 μg/mL (r=0.9998) and 0.418-8.352 μg/mL (r=0.9999),respectively.The average recoveries (n=6) were 99.55% (RSD=1.74%),98.86% (RSD=1.74%) and 99.97% (RSD=1.70%).Total flavonoids were determined by UV spectrometry.The linear range of baicalein was 1.71-8.54 μg/mL (r=0.9991).The average recoveries (n=6) were 100.93% (RSD=2.63%).The contents of baicalein,wogonin,oroxylin A and total flavonoids were 48.22%-60.36%,12.09%-14.46%, 5.05%-8.81% and 78.11%-90.12%,respectively.

Key words: Scutellaria Radix total flavone aglycone extract, baicalein, wogonin, oroxylin A, ultraviolet, high performance liquid chromatography, content determination

中图分类号: 

R284.2

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