天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (9): 1595-1600.doi: 10.16333/j.1001-6880.2015.09.015

• 研究简报 • 上一篇    下一篇

响应面法优化马兰总三萜提取工艺研究

王国凯,王诤,蔡百祥,高卫娜,刘劲松,王刚*   

  1. 安徽中医药大学药学院 现代中药安徽省重点实验室,合肥 230012
  • 出版日期:2015-09-25 发布日期:2015-09-28

Optimization of Extraction of Triterpenes from Kalimeris indica by Response Surface Methodology

WANG Guo-kai, WANG Zheng, CAI Bai-xiang, GAO Wei-na, LIU Jin-song, WANG Gang*   

  1. School of Pharmacy,Anhui University of Chinese Medicine & Anhui Key Laboratory for modern Chinese Materia Medica,Hefei 230012,China
  • Online:2015-09-25 Published:2015-09-28

摘要: 利用响应面法优化马兰总三萜的最佳提取工艺。以乙醇浓度、液料比、提取时间、提取次数为主要考察因素进行单因素试验,并分析各因素对马兰总三萜提取效率的影响。在此基础上,设计响应曲面试验,从而优化马兰总三萜的提取工艺。实验结果显示最佳提取工艺为乙醇浓度40%,料液比1:18 g/mL,提取时间1 h,提取次数4次。此最佳提取工艺所得到的马兰总三萜的含量为26.83 mg/g。说明此方法的提取率较高,提取工艺稳定。

关键词: 马兰, 总三萜, 提取工艺, 响应面法

Abstract: The objective of this study was to optimize the extraction of triterpenes from Kalimeris indica through response surface methodology.Based on single factor tests, Box-Behnken central composite design was applied to investigate the effects of ethanol concentration,solid/liquid ratio,ultrasonic duration,times of ultrasonic on the extraction yield of triterpenes and hence to optimize these extraction conditions.By means of regression analysis,the results showed that the optimized conditions were:ethanol concentration of 40%,solid/liquid ratio of 1:18 g/mL,extraction duration of 1 h and with 4 times of extraction.Under these conditions,the extraction yield of triterpenes reached 26.83 mg/g.The result indicated that the extraction system was efficient and reliable.

Key words: Kalimeris indica, extraction of triterpenes, extraction technology, response surface methodology

中图分类号: 

R284.2