天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (10): 1723-1727.doi: 10.16333/j.1001-6880.2015.10.007

• 研究论文 • 上一篇    下一篇

吴茱萸碱不同加药时序对胃癌SGC7901细胞放射敏感性的影响

汉媛媛1,2,张凯亮1,高雪翔1,李洁1,3,郭琪1,Juraev Firdavs1,王静1*   

  1. 1 兰州大学口腔医学院,兰州 730000;2 甘肃省妇幼保健院,兰州 730050;3 天津高等医学专科学校,天津 300222
  • 出版日期:2015-10-30 发布日期:2015-10-30

Radiosensitizing Effects of Evodiamine with Different Time Series of Administration on Human Gastric Cancer Cells

HAN Yuan-yuan 1,2, ZHANG Kai-liang1, GAO Xue-xiang1, LI Jie 1,3, GUO Qi 1, Juraev FIRDAVS1, WANG Jing 1*   

  1. 1 School of stomatology,Lanzhou University,Lanzhou 730000,China; 2 Gansu Maternity and Child  Care Centers,Lanzhou 730050,China; 3 Tianjin Medical College,Tianjin 300222,China
  • Online:2015-10-30 Published:2015-10-30

摘要: 在前期实验证实吴茱萸碱(Evodiamine,EVO)对舌鳞状细胞癌Tca8113细胞有放射增敏的作用,为进一步研究EVO作为放射增敏剂应用的可能性,并探讨不同加药时序对放射增敏作用的影响是否有差异,采用EVO联合射线作用于胃癌SGC7901细胞探讨EVO对其放射敏感性的影响。采用MTT法检测不同时间点、浓度EVO对SGC7901细胞增殖的抑制率,并检测低浓度EVO不同加药顺序联合射线后对SGC7901细胞增殖的抑制率;克隆形成实验分析联合作用对SGC7901细胞克隆形成能力的影响,拟合存活曲线并计算各组细胞放射增敏比;流式细胞术分析EVO联合放射线作用后细胞周期分布变化;Western blot检测凋亡相关蛋白caspase3的表达水平。通过上述实验方法得到EVO对SGC7901细胞的生长抑制作用呈时间、剂量依赖,并且不同加药顺序对细胞增殖的抑制效果不同(P<0.05)。克隆形成实验中求得联合作用组各放射剂量下的存活分数、放射生物学敏感参数均低于单独放射组,且联合作用组中先加药组的增敏效果优于后加药组(P<0.05)。流式细胞术检测EVO联合不同放射剂量使细胞周期阻滞在G2/M期的比率明显高于单独放射组及单独加药组(P<0.05)。Western blot结果提示联合作用组中凋亡蛋白caspase3的表达高于单独放射组及单独加药组(P<0.05)。综上所述,EVO对SGC7901细胞有放射增敏作用,并且不同加药顺序对放射增敏作用的影响不同,联合作用中先加药后放射的效果优于先放射后加药组,其增敏效果的作用机制可能与联合作用导致细胞G2/M期阻滞、凋亡蛋白caspase3表达增加有关。

关键词: 吴茱萸碱, 放射增敏, 胃癌, 细胞周期, caspase3

Abstract: Preliminary experiments confirmed that evodiamine (EVO) has radiosensitizing effect on the tongue squamous cell carcinoma cell line Tca8113.We wonder the efficacy of EVO combined radiotherapy with different treatment schedules on human gastric cancer cells SGC7901.Cell proliferation inhibitory rates of SGC7901 cells were detected by MTT method at different time points after treatment with EVO at different concentrations.The colony-formation ability of SGC7901 cells treated with EVO plus radiation was detected by colony-formation assay.Cell cycle was detected to compare the difference between radiotherapy and EVO with different doses of radio-therapies by flow cytometry.Western blot assay was conducted to determine the expression of Caspase3 in the cell lines.The grouping of EVO administration was as follows:Group A,administration 24 h before irradiation;Group B,administration after irradiation;inhibition rates of SGC7901 cells were increased as EVO increased in concentration and time.Survival fraction,D0, Dq, SF2 of combination groups were lower than that in the radiation and combination groups.The effect of Group A was superior to that of Group B.G2/M blockage rates of the EVO in combination was higher than other groups.The protein expression of caspase3 in the combination group was markedly increasing compared with radiation group.The radiosensitizing effect was affected by different time series of administration,in which the effect of the administration before radiotherapy ranked the first.The mechanism of radiosensitization effect may relate to G2/M cell cycle arrest and increase expression of Caspase3.

Key words: evodimine, radiosensitization, gastric cancer, cell cycle, caspase3

中图分类号: 

R73