关键词: 四棱豆, 叶, 花, 挥发性成分, 顶空固相微萃取, 气相色谱-质谱

Abstract: The volatile components in leaves and flowers of Psophocarpus tetragonolobus (Linn.) DC.were extracted by headspace solid phase microextraction for the first time,and their chemical components were identified by GC-MS and relative content of the components were calculated with peak area normalization method.The results showed that 32 compounds,representing 65.60% of the total of volatile compounds,were identified from the leaves.The main components were heptadecane (5.75%),2-methyl-hexadecane (5.29%),pristane (5.05%),3-methyl-hexadecane (4.26%),palmitic acid (3.76%),2-bromo-dodecane (3.14%),hexahydrofarnesylacetone (2.95%),dihydroactinidiolide (2.89%) and myristicin (2.59%),and was mainly composed of alkanes (38.25%),ketones (8.49%),acids (3.76%),esters (6.76%) and ethers (3.51%).33 compounds were identified from flowers,which comprised 76.91% of the volatile fraction.The main components were plamitic acid (11.42%),hexahydrofarnesylacetone (10.77%),heptadecane (4.79%),hexadecane (4.69%),myristicin (3.88%),pristane (3.42%),2-bromo dodecane (3.10%),3-methyl-hexadecane (2.97%),oleic acid (2.50%) and hexanoic acid (2.43%),and was mainly composed of alkanes (29.42%),ketones (13.29%),acids (19.51%),esters (5.89%) and ethers (4.71%).23 compounds were common in both parts of P.tetragonolobus with different quantities.These ingredients gave different pharmacological activity between leaves and flowers of P.tetragonolobus.

Key words: Psophocarpus tetragonolobus (Linn.) DC., leaves, flowers, volatile constituents, HS-SPME, GC-MS