淫羊藿素促进BMSCs成软骨分化的研究

doi:10.16333/j.1001-6880.2015.11.006

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (11): 1866-1870.doi: 10.16333/j.1001-6880.2015.11.006

淫羊藿素促进BMSCs成软骨分化的研究

汪建样1,2 ，殷嫦嫦3，王子瑶1,2，耿书国1,2，胡文龙1,2，殷明1*

1 南昌大学第二附属医院；2 南昌大学研究生院医学部，南昌 330006；3 九江学院，九江 332000

出版日期:2015-11-30 发布日期:2015-12-02

Icaritin Promotes Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

WANG Jian-yang1,2, YIN Chang-chang3, WANG Zi-yao1,2, GENG Su-guo1,2, HU Wen-long1,2, YIN Ming1*

1 Department of Orthopedics,the Second Affiliated Hospital of Nanchang University;2 Medicine Graduate School,Nanchang University, Nanchang 330006,China;3 Basic Medical College,Jiujiang University,Jiujiang 332000,China

Online:2015-11-30 Published:2015-12-02

摘要/Abstract

摘要： 研究淫羊藿素在GDF-5诱导BMSCs成软骨分化过程中的作用。全骨髓贴壁法分离培养SD大鼠骨髓间充质干细胞（BMSCs），取P3代细胞随机分成4组：对照组，淫羊藿素（Icaritin）组，Growth differentiation factor 5（GDF-5）组，Icaritin+GDF-5联合组。连续诱导培养14 d，倒置相差显微镜观察细胞形态，Alcian Blue染色检测细胞的蛋白聚糖改变，RT-PCR检测软骨分化标记基因Aggrecan、COL2、Sox9及COL1的表达情况，Western Blot检测COL2和COL1蛋白表达水平。结果提示，与对照组及GDF-5组相比，Icaritin+GDF5联合组蛋白聚糖染色更深；软骨分化标记基因Aggrecan、COL2、Sox9明显增加；Ⅱ型胶原蛋白表达量均明显增加。淫羊藿素能够促进GDF-5诱导BMSCs成软骨分化。

关键词: 淫羊藿素, GDF-5, BMSCs, 成软骨分化

Abstract: In this study,the effects of icaritin on chondrogenic differentiation of bone marrow mesenchymal stem cells induced by growth differentiation factor 5 were investigated.BMSCs were collected from Sprague Dawley rats.The morphology was observed by inverted microscope.rBMSCs at passage 3 were randomized divided into 4 groups:control group,Icaritin group,GDF-5 group,Icaritin+GDF-5 group and induced for 14 days.Alcian Blue stain showed the changes of proteoglycans;RT-PCR was used to detect the mRNA expression of Aggrecan,COL2,Sox9 and COL1;the protein expression levels of COL2 and COL1 were detected by Western Blot assay.The results indicated that,compared with the control group and GDF-5 group,Icaritin +GDF-5 group showed deeper proteoglycans staining;cartilage differentiation marker genes Aggrecan,COL2,Sox9 and the protein expression levels of COL2 significantly increased.Icaritin can promote chondrogenic differentiation of bone marrow mesenchymal stem cells induced by GDF-5.

Key words: icaritin, GDF-5, BMSCs, chondrogenic differentiation

中图分类号:

R963

汪建样1,2 ，殷嫦嫦3，王子瑶1,2，耿书国1,2，胡文龙1,2，殷明1*. 淫羊藿素促进BMSCs成软骨分化的研究[J]. 天然产物研究与开发, 2015, 27(11): 1866-1870.

WANG Jian-yang1,2, YIN Chang-chang3, WANG Zi-yao1,2, GENG Su-guo1,2, HU Wen-long1,2, YIN Ming1*. Icaritin Promotes Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(11): 1866-1870.

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淫羊藿素促进BMSCs成软骨分化的研究

Icaritin Promotes Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

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