关键词: 棘托竹荪, 菌托, 抑菌活性物质, GC-MS

Abstract: Components from volva of Dictyophora echinovolvata were extracted by different extraction solvent and their antimicrobial effects were determined using Staphylococcus aureus, Bacillus subilis, Escherichia coli, Bacillus cereus, Morganella fulton, Salmonella enteritidis as indicator bacteria．In addition,the components with highest antibacterial activity were separated by column chromatography and the purified fractions were determined by gas chromatography-mass spectrometry (GC-MS).Results showed that the ethyl acetate extract from volva had highest antibacterial effect on the above pathogenic bacteria and was more stable for temperature and ultraviolet radiation.The ethyl acetate extract was then further purified to afford 2 antibacterial components,namely DEV I and DEV II.Through GC-MS analysis,twenty-six components with the relative content of more than 1% were identified in DEV I and DEV II,respectively.The major antibacterial components of DEV II were 2-furoic acid with relative contents of 25.6% and cinnamic acid with relative contents of 54.2%.These two components were possibly the major antibacterial components from volva of D.echinovolvata.

Key words: Dictyophora echinovolvata, volva, antibacterial components, GC-MS