关键词: 五味子, 过氧化氢, 氧化应激损伤, HaCaT细胞

Abstract: In this study,the protective effects of different fractions of Schisandra chinensis Fructus on H₂O₂-induced oxidative stress injury in HaCaT cells were investigated and the active components were screened.The extracts of S.chinensis fruits were separated with macroporous resin.With DPPH free radical scavenging activity and total antioxidant capacity as indexes,the antioxidant activities of S.chinensis were assessed.MTT method was used to screen the protective effects of different fractions of S.chinensis on H₂O₂-induced oxidative stress injury in HaCaT cells.The active components with better protective effect were obtained.The results showed that 50% EtOH fraction demonstrated the strongest antioxidant activity and can inhibit the H₂O₂-induced injury in HaCaT cells.Furthermore,50% EtOH fraction reduced MDA activity and elevated SOD,GSH activities.The results suggested that 50% EtOH fraction was the active components of S.chinensis protecting skin cells against H₂O₂-induced oxidative stress injury.

Key words: Schisandra chinensis, hydrogen peroxide, oxidative stress injury, HaCaT cell