天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (12): 2035-2039.doi: 10.16333/j.1001-6880.2015.12.007

• 研究论文 • 上一篇    下一篇

香椿子石油醚提取物对大鼠糖尿病肾病的保护作用

李万忠*,王晓红,韩玮娜,刘冬梅   

  1. 潍坊医学院药学院,潍坊 261053
  • 出版日期:2015-12-29 发布日期:2016-01-06

Protective Effect of Petroleum Ether Fraction of Toona sinensis Roem Seeds Extracts on Kidney of Diabetic Nephropathy Rats

LI Wan-zhong*, WANG Xiao-hong, HAN Wei-na, LIU Dong-mei   

  1. College of pharmacy,Weifang Medical University,Weifang 261053,China
  • Online:2015-12-29 Published:2016-01-06

摘要: 为了研究香椿子石油醚提取物(PEE)对糖尿病肾病(DN)大鼠的保护作用及初步机制,采用Wistar雄性大鼠,STZ 60 mg/kg造模。造模成功后,DN大鼠分为模型组、PEE干预组(5 mg/100 g·d),另设正常组。灌胃10周后处死,取材及采血,检测肾脏指数及生化指标;肾皮质行HE和PAS、PASM、Masson染色;电镜观察大鼠肾组织形态;免疫组化观察转化生长因子-β1(TGF-β1)、结缔组织生长因子(CTGF)、IV型胶原蛋白(collagen IV)表达水平。结果显示,PEE组大鼠血糖、尿蛋白、氧化应激指标下降;血肌酐与尿素氮降低,明显改善DN大鼠肾脏病理学异常,降低肾脏TGF-β1、CTGF、collagen IV蛋白表达。这表明PEE抑制氧化应激,减少TGF-β1、CTGF、collagen IV蛋白表达,对DN大鼠有一定保护作用。

关键词: 香椿子石油醚提取物, 糖尿病肾病, 氧化应激, 免疫组化

Abstract: The objective of this study was to observe the protective effect of petroleum ether fraction of Toona sinensis Roem seeds extracts on the kidney of diabetic nephropathy (DN) rats and its preliminary mechanism.Male Wistar rats were fed up with high-fat diet for one month.DN rats were established by injecting streptozotocin at the dose of 60 mg/kg.DN rats were divided into model group,sample intervention group (5 mg/100g·d) and normal control group.After treatment for 10 weeks,the rats were sacrificed,the blood samples and the renal cortex were collected.Biochemical indicators in serum and renal index were determined. HE staining,PAS staining,PASM staining and Masson staining were applied for observing the pathological changes of the renal tissues.Changes of renal structure were observed in DN rats by transmission electron microscopy.The protein levels of TGF-β1,CTGF and collagen IV were detected.The petroleum ether fraction of T.sinensis seeds extracts can reduce blood glucose,urinary albumin,renal index,oxidative stress index,serum creatinine and urea nitrogen.The renal pathology abnormality was improved in DN rats.The protein levels of TGF-β1,collagen IV and CTGF were increased.The expressions of TGF-β1,collagen IV and CTGF were decreased.These results indicated that petroleum ether fraction of T.sinensis seeds extracts had protective effects on DN rats by inhibiting oxidative stress,protein expression of TGF-β1,collagen IV and CTGF.

Key words: petroleum ether fraction of Toona sinensis seeds extract, diabetic nephropathy, oxidative stress, immunohistochemistry

中图分类号: 

R285.5