天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (12): 2090-2094.doi: 10.16333/j.1001-6880.2015.12.018

• 研究简报 • 上一篇    下一篇

组培霍山石斛、野生霍山石斛及河南石斛多糖及乙醇溶出物动态积累规律研究

陈乃东1,2,3*，陈乃富1,2，王陶陶1,2，顾镇1,2   

  1. 1 皖西学院生物与制药工程学院；2 皖西中药与天然药物工程技术研究中心，六安 237012；3 安徽医科大学药学院，合肥 230032
  • 出版日期:2015-12-29 发布日期:2016-01-06

Accumulation of Polysaccharides and Ethanol soluble Extract in Tissue-cultured and Wild Dendrobium huoshanense and Dendrobium henanense

CHEN Nai-dong 1,2,3*, CHEN Nai-fu1,2, WANG Tao-tao 1,2, GU Zhen 1,2   

  1. 1 College of Biotechnology and Pharmaceutical Engineering,West Anhui University; 2 West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine,West Anhui University,Lu’an 237012,China; 3 School of Pharmacy,Anhui Medical University,Hefei 230032,China
  • Online:2015-12-29 Published:2016-01-06

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摘要： 为了揭示不同种源的霍山石斛及其近缘种河南石斛多糖和醇溶性浸出物含量的动态变化规律，为霍山石斛和河南石斛规范化种植及品种鉴定提供依据，采用蒽酮-浓硫酸比色法和醇溶性浸出物热浸法对1~4年株龄的野生霍山石斛、组培霍山石斛和河南石斛的多糖和浸出物含量进行测定。结果表明，不同株龄的组培霍山石斛、野生霍山石斛和河南石斛的多糖、浸出物含量均存在极显著差异。以多糖含量为标准，三者均以生长2年采收为宜，以醇溶物为标准，以栽培三年采收最好。以多糖和醇溶出物总量计，组培霍山石斛以2年采收为宜，野生霍山石斛及河南石斛以3年株龄品质最佳。

关键词: 组培霍山石斛, 野生霍山石斛, 河南石斛, 多糖, 乙醇浸出物

Abstract: In order to reveal the accumulation of pharmaceutical constituents in different origins of Dendrobium huoshanense and its main counterfeit herb Dendrobium henanense, and to provide references for their standardized planting and cultivar identification,the contents of polysaccharides and ethanol-soluble extract in different ages of tissue-cultured and wild D.huoshanense as well as D.henanense were detected by anthrone-sulfuric acid colorimetric and hot-maceration method,respectively.The results showed that the accumulation of polysaccharides and ethanol-soluble extract in the 12 Dendrobium samples varied remarkably as their ages increased.It was better to harvest when they were cultivated for two years according to their polysaccharide contents.It was better to harvest when they were cultivated for three years according to their ethanol-soluble extract contents.It was more reasonable to harvest tissue-cultured D.huoshanense cultivated for two years while it was better to harvest wild D.huoshanense and D.henanense cultivated for 3 years according the total contents of polysaccharides and ethanolsoluble extract.

Key words: tissue-cultured Dendrobium huoshanense, wild Dendrobium huoshanense, Dendrobium henanense;polysaccharide;ethanol-soluble extract

中图分类号: 

R932

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