天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (Suppl): 113-116.doi: 10.16333/j.1001-6880.2015.S.028

• 开发研究 • 上一篇    下一篇

桑葚和葡萄籽不同提取物对PC12细胞的影响

李鑫,李林竹,付正,李景明*   

  1. 中国农业大学食品科学与营养工程学院,北京 100083
  • 出版日期:2015-12-01 发布日期:2016-01-12

Effect on the Survival Rate of PC12 Cells from Extract of Mulberries and Grape Seeds

LI Xin,LI Lin-zhu,FU Zheng,LI Jing-ming*   

  1. China Agricultural University College of Food Science and Nutrional Engineering,Beijing 100083,China
  • Online:2015-12-01 Published:2016-01-12

摘要: 为研究桑葚和葡萄籽等食源性材料潜在的功能活性,比较其不同提取物对PC12细胞的作用,本实验采用不同极性的溶剂(水、乙醇、乙酸乙酯、正己烷)对桑葚和葡萄籽进行提取,测定不同提取物对PC12细胞的影响。结果表明,葡萄籽提取物对PC12细胞的影响强于桑葚,其水提取物和乙醇提取物对PC12细胞有较大的促进生长作用,作用浓度在50~500 mg /L作用时间为4 h时对PC12细胞的存活率有最为显著的提高。因此葡萄籽水提取物和乙醇提取物具有潜在的细胞保护作用,可为进一步的开发提供理论依据。

关键词: PC12细胞, 桑葚提取物, 葡萄籽提取物, 共培养

Abstract: In order to study the foodborne material which have potential functions such as grape seeds and mulberries,comparing their different extracts on PC12 cells.The mulberries and grape seeds were extracted by different polar solvents (water,ethanol,ethyl acetate,n-hexane),measuring the effects of different extracts on PC12 cells.The results showed that the grape seeds extract influencing PC12 cells more than the mulberries.When the grape seeds water extract and ethanol extract tested in 50-500 mg/L,4h,the survival rate of PC12 cells had the most significant improvement.In consequence,grape seeds water extract and ethanol extract have the potential of cell protection,which can provide a theoretical basis for further experiment.

Key words: PC12 cells, mulberry extract, grape seed extract, coculture

中图分类号: 

TS201.2