Abstract: Thirteen compounds were isolated and purified from the ethanolic extracts of pine cone of Pinus yunnanensis by MPLC and column chromatography on silica gel,RP-18,MCI,and Sephadex LH-20.On the basis of spectroscopic analysis,the isolated compounds were identified as 16-nor-15-oxodehydroabietic acid (1), 16-nor-7,15-dioxodehydroabietic acid (2), 7α,15-dihydroxydehydroabietic acid (3), methyl 15-hydroxy-7-oxodehydroabietate (4),imbricatolic acid (5), isokobusone (6), alternariol (7),matairesinol (8),(+)-epipinoresinol (9),taxifolin 3′-O-glucoside (10),pinosylvin (11),(Z)-3-hydroxy-5-methoxystilbene (12) and coniferaldehyde (13).All compounds were obtained from P.yunnanensis for the first time and compounds 1,2,6,7 and 9 were obtained from the genus Pinus for the first time.

Key words: Pinus yunnanensis, Pinaceae, pine cone, chemical constituents, abietane diterpenoid