NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (2): 214-220.doi: 10.16333/j.1001-6880.2015.02.004

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Determination of 4 Tritepenoidic Acids in Pomegranate by Precolumn Fluorescent Derivatization Coupled with HPLC-FLD

YUAN En-guang1, LI Guo-liang1,3*, WU Hong-liang2, LIU Shu-cheng3, XIA Lian1, YOU Jin-mao1,2*, SUO You-rui2   

  1. 1 Key Laboratory of Life Organic Analysis of Shandong Province,Qufu Normal University,Qufu 273165,China; 2 Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China; 3 College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution,Zhanjiang 524088,China
  • Online:2015-02-15 Published:2015-02-15

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Abstract: In this study,a fast and sensitive HPLC method has been developed for the determination of triterpenic acids using 2-(7H-dibenzo［a,g］carbazol-7-yl)ethyl 4methylbenzenesulfonate (DBCETS) as the fluorescent labeling reagent.A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling including labeling time,reaction temperature and the concentration of labeling reagent.Under the optimized conditions,the proposed method gave good reproducibility and accuracy.The linear correlation coefficients for all triterpenic acid derivatives were of >0.9995,and the limit of detections were in the range of 1.10~1.48 ng/mL (S/N=3).The proposed method has been firstly applied for the determination of corosolic acid,maslinic acid,oleanolic acid and ursolic acid in pomegranate,and got satisfactory results.

Key words: pomegranate, HPLC, maslinic acid, oleanolic acid, ursolic acid, corosolic acid

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