Abstract: To determine morroniside in Fructus Corni rapidly by near infrared spectroscopy(NIRS).HPLC method was used as a reference method to determine the content of morroniside in Fructus Corni.NIR spectra of the powdered Fructus Corni were collected,and the PLS was developed to correlate the spectra and the corresponding HPLC values.The correlation coefficient (R²) of the calibration model of NIR spectroscopy for morroniside was 0.9845,the root-mean-square error of crossvalidation(RMSECV),the correction of deviation,the prediction mean square error was 0.109,0.2641 and 0.233,respectively.The parameters of the model were also discussed.The overall results showed that NIR spectroscopy combined with PLS can be efficiently utilized for rapid and accurate analysis of morroniside in Fructus Corni.In conclusion,the developed NIRS combined TQ software analysis method was rapid,accurate,nondestructive and no pollution in the quality control of traditional Chinese Medicine(TCM).

Key words: near-infrared spectroscopy, partial least square, Fructus Corni, morroniside, quantitative model