Abstract: The flavonoids were extracted from Pyracantha fortuneana with ultrasonic-assisted ethanol extraction and purified through D-101 macroporous resin to afford crude extracts (PFF1) and purified flavonoids (PFF2),respectively.The content of PFF1 and PFF2 were determined using rutin as index.The antioxidant activity of PFF1 and PFF2 were investigated by testing the peroxidation and acid value of oils and fats,and compared with V_E.The results showed that under the experiment conditions,the flavonoids mass concentration of PFF1 and PFF2 were 2.25 g/L and 3.49 g/L,respectively.The extraction rate was 5.11% and the purity rate was 22.84%.At the flavonoids mass concentration of 0.258 g/L,the protection rate to oils and fats from rapeseed,olive,goose,chicken,pork suet,beef tallow were 27.56%,19.66%,16.12%,24.05%,24.06%,20.61% for PFF1,and 46.17%,38.15%,37.15%,42.31%,32.78%,28.43% for PFF2,respectively.ΔAVof oils and fats were 0.68,0.56,0.44,0.8,0.48,0.28 mg/g for PFF1,and 1.0,0.6,0.56,0-96,0.52,0.36 mg/g for PFF2,respectively.In the range of 0.040.26 g/L,the antioxidant effect of PFF1 and PFF2 increased with the flavonoids mass concentration increasing.In the range of 50-80 ℃,the antioxidant effects of PFF1 and PFF2 increased with the temperature rising.Under the same concentration,the antioxidant activity of PFF2 was higher than that of PFF1,but lower than that of V_E.

Key words: Pyracantha fortuneana, flavonoids, peroxide value, acid value, antioxidant activity