Abstract: The aim of this study was to investigate the protective effect of total glycosides from Cistanche salsa on mice alcoholic liver injury according to serum index and histopathology of liver.The three groups of total glycosides of C.salsa were respectively high dose (130 mg/kg),middle dose (100 mg/kg),low dose (65 mg/kg) and bifendate pills (150 mg/kg) was used as positive control.The results showed that ALT,AST and MDA were increased and SOD was decreased in model mice.Besides,the fatty degeneration was also observed in the hepatic cells of model mice.However,high doses of C.salsa total glycosides can effectively improve the liver function.The contents of echinacoside and verbascoside in C.salsa total glycosides were 29.1% by HPLC.In conclusion,total glycosides of C.salsa had a protective effect on mice alcoholic fatty liver injury,and its main active components were echinacoside and verbascoside.

Key words: total glycosides of Cistanche salsa, HPLC, verbascoside, echinacoside, alcoholic liver injury