Abstract: In this study,α-GSH and γ-GSH were synthesized by Fmoc solid phase peptide synthesis method,using 2-chlorotrityl chloride (2-CTC) resin as solid carrier and DIC/HOBt as condensing agents.The protected peptide was obtained by cleavage with TFA/EDT/m-Cresol.The crude GSSG peptide was then formed by air,H₂O₂ or I₂ oxidation of γ-GSH separately.After semi-preparative RP-HPLC purification,three pure peptides were obtained.The purities of α-GSH,γ-GSH,GSSG pure peptide were 99%,99%,98%,and the total yields were 60%,64%,57%,respectively.Finally,their hepatoprotective effect on CCl₄-induced acute liver injury in mice was observed.The result showed that three synthetic pure peptides can significantly decrease ALT and AST activities in mice serum,and there were no significant differences with γ-GSH for injection,which may provide reference for industrial production.

Key words: glutathione, Fmoc solid phase peptide synthesis, disulfide bond formation, biological activity