Abstract: Nine known compounds were isolated from Aspergillus oryzae RER4,and their structures were elucidated as ergosterol (1),5-hydroxy-benzofuran-2 (3H)-one (2),2,5-di-hydroxyphenyl acetate (3),5-hydroxymethylfuran-3-carboxylic acid (4),pyranonigrins A (5),campyrones A (6),4-acetamidobutanoic acid (7),rubrofusarin B (8) and asperpyrones A (9) by comprehensive spectral analysis.Compound 2-9 were isolated from the culture of A.oryzae for the first time.The DPPH radicalscavenging activity assays showed that compounds 2 and 3 had potential activity with IC₅₀ of 4.58 and 7.00 μg/mL,respectively.In the antimicrobial activity test for four bacteria (E.coli, P.aeruginosa, S.aureu, B.subtilis) and four fungi (F.oxysporium, G.saubinerii, C.mandshurica, B.cirerea),all compounds exhibited a wide inhibition to bacterial and fungal growth.

Key words: endophytic fungus, Lycium ruthenicum Murr, metabolites, bioactivities