DPPH Free Radical Scavenging Efficiency of Total Flavonoids from Scorzonera divaricata

doi:10.16333/j.1001-6880.2015.09.019

Abstract

Abstract: To investigate the antioxidant activity of total flavonoids in Scorzonera divaricata, microwave-assisted extraction method was used to extract total flavonoids. Antioxidant activity was measured by DPPH free radial scavenging assay.Meanwhile,the dynamic model of response curves was simulated by Graphpad Prism 5.0 software.The results showed that the total flavonoids had higher scavenging activity on DPPH free radial than Vc and rutin,clearance rate was 41.72%-87.72%, IC₅₀ was 5.6 μg/mL.A kinetics equation was established:Y=(Y0-NS)*exp(-K*X) + NS,which very well reflected the efficiency of free radical scavenging.The appropriate concentration of the sample was 20 μg/mL.

Key words: Scorzonera divaricata, total flavonoids, DPPH free radical, kinetics of reaction

CLC Number:

MA Gou-cai1,2, ZHAO Li-na1, BAI Hong-jin2*. DPPH Free Radical Scavenging Efficiency of Total Flavonoids from Scorzonera divaricata[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(9): 1616-1619.

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DPPH Free Radical Scavenging Efficiency of Total Flavonoids from Scorzonera divaricata

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