NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (1): 112-119. doi: 10.16333/j.1001-6880.2016.1.020

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Infrared Spectroscopy Analysis and Pharmacological Activity of Ethanol Extracts of Wild and Cultivated Swertia mussotii Franch

YANG Hong-xia1,2,LI Cen1,2,GAO Ting-ting1,2,GENG Lu-jing1,2,3,WEI Li-xin1,2, DU Yu-zhi1,2*   

  1. 1 Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Qinghai key laboratory of Tibetan Medicine Pharmacology and Safety Evaluation; 2 Key  Laboratory of Tibetan Medicine Research,Chinese Academy of Sciences,Xining 810008,China; 3University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2016-01-29 Published:2016-02-01

Abstract: To compare the ingredient and pharmacological activity of ethanol extracts of wild and cultivated Swertia mussotii Franch. The method of HPLC and infrared spectroscopy was used to compare the ingredient of extracts of wild and cultivated S. mussotii Franch;liver injury model induced by CCl4 in mice,acetic acidinduced writhing experiment and normal mouse were used to compare the hepatoprotective function,abirritation and peristaltic inhibition of the extracts. The total content of swertiamarin,gentiopicrin and mangiferin of wild and cultivated S. mussotii Franch were 3.949% and 4.539% respectively;Infrared spectroscopy of ethanol extracts had no obvious difference,while two-dimensional correlation spectroscopy (2D-COS) of them had evident difference. The pharmacological experiments results proved their hepatoprotective function,abirritation and peristaltic inhibition,while the enhancement pharmacological activity of cultivated sample with the increasing of dosage was more obvious when it compared with that of wild sample.

Key words: Swertia mussotii Franch, wild, cultivated, infrared spectroscopy, pharmacological activity

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