Isolation and Identification of Secondary Metabolites from the  Solid Culture of Endophytic Fungal Strain Pestalotiopsis uvicola GMH31 from Artemisia japonica

doi:10.16333/j.1001-6880.2016.11.008

Abstract

Abstract: Ten chemical constituents were isolated and purified from the solid culture of endophytic fungal strain Pestalotiopsis uvicola GMH31 isolated from Artemisia japonica by silica gel column chromatography,Sephadex-LH20 column chromatography and recrystallization.On the basis of spectral data and physicochemical properties,their structures were identified as thymine (1),uracil (2),luteolin (3),rutin (4),quercetin (5),vanillic acid (6),protocatechuic acid (7),kaempferol (8),genistein (9) and 4′,5,7-trihydroxyflavanone (10).All the compounds were isolated from P.uvicola for the first time,and compound 3~5 and 8~10 belong to flavonoids.Compound 8 showed better reversal effect on the ovarian cancer cell line A2780/Taxol.

Key words: Pestalotiopsis uvicola, endophytic fungus, secondary metabolite, Artemisia japonica

CLC Number:

R284.1

QIAN Yi-xin1,KANG Ji-chuan1,2*,HE Jun1,WANG Lu1,LEI Bang-xing1. Isolation and Identification of Secondary Metabolites from the Solid Culture of Endophytic Fungal Strain Pestalotiopsis uvicola GMH31 from Artemisia japonica[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(11): 1732-1735.

[1]	SHENG Meng-ke, ZHAO Ying-fei, TIAN Ruo-dan, WEN Chun-nan, LIU Miao, MA Bing-ji. Secondary metabolites from the endophytic Chaetomium sp. of Dioscorea opposita [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(6): 982-985.
[2]	ZHANG Meng-jiao, KUANG Tian-hao, ZHAO Jin-nuo, ZHU Ting, PENG Juan-juan. Secondary metabolites of endophytic fungus Septoriella phragmitis from Eupatorium chinense and its bioactivity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(6): 986-992.
[3]	CAO Wen-ying, XU Bing-yan, PENG Guo-qing, CHEN Yin, HUANG Jian-ping, HUANG Sheng-xiong. Research progress of secondary metabolites based on Astragalus membranaceus hairy root technique#br# #br# [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊2): 164-173.
[4]	SONG Guo-dong, LIAO Hong-juan, ZHANG Zhi-bin, ZHU Du. Advances in non-directed activation of silent gene clusters for mining active secondary metabolites of Streptomyces [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(8): 1442-1456.
[5]	ZENG Qing-ting, YUAN Tao. Secondary metabolites of endophyte Paraconiothyrium sp.YLHJ01 from Artemisia selengensis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(5): 781-786.
[6]	DAI Ying, YANG Fan, LIN Hou-wen . Research progress on secondary metabolites and pharmacological activities of polar actinomycetes [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(5): 901-913.
[7]	LIAO Hong-juan, ZHANG Zhi-bin, JIANG Yu-mei , ZHU Du. Research progress of microbial secondary metabolites against Phytophthora capsici [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(4): 705-721.
[8]	TAN Hui, PI Li, YANG Xiao-long, AN Ting, GUO Xu-dong, LIU Yan-ping, WEN Huai-xiu. Isolation and indentification of an endophytic fungus X-396 from Hypecoum leptocarpum Hook.f.et Thoms.and analysis on fat-soluble components of its fermentation [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(3): 420-426.
[9]	ZHANG Kuan-chao, XU Chen-yang, YU Jia-qi, SU Hong-cheng, XU Zhong-you. Extraction and purification of total flavonoids from Artemisia japonica Thunb.and its antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(2): 281-290.
[10]	WU Yu-zhen, ZHANG Hui-min, AN Fang-fang, ZHENG Yong-biao. Research progress on secondary metabolites of Aspergillus versicolor from different habitats [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(2): 342-352.
[11]	LI Si-min, ZHOU Jin-cai, HE Rong, TANG Ying-nan, ZHOU Si-qian, WANG Ya-jing, LONG Hong-ping. Study on the secondary metabolites from marine-derived fungi Aspergillus terreus [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(10): 1732-1738.
[12]	WU Shou-yuan, ZENG Ling, SUN Yu-lin, CHEN Zi-ming. Study on secondary metabolites of fungus Penicillium janthinellum F12 from the South China Sea [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(增刊2): 48-52.
[13]	WANG Yi, JIN Tong, ZHAN Yi-cong, ZHANG Hua-wei. Advance in new secondary metabolites from the genus Aspergillus using OSMAC strategy [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(增刊1): 173-181.
[14]	ZHEN Jin-cheng, SI Lu, YU Hong-jia, DU Ting-ting, LIU Yao, SHAN Ti-jiang, XU Li-jian. Study on secondary metabolites and antimicrobial activities of three fungi from forest litter in the Greater Hinggan Mountains [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(9): 1515-1522.
[15]	CHEN Lin, WANG Min, HU Yuan, LIU You-ping, CHEN Hong-ping, WANG Fu. Differential analysis of secondary metabolites of medicinal and edible Polygonati Rhizoma and study on potential active components and mechanism for improving insulin resistance [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(9): 1582-1596.

Isolation and Identification of Secondary Metabolites from the Solid Culture of Endophytic Fungal Strain Pestalotiopsis uvicola GMH31 from Artemisia japonica

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments