Abstract: The aim of this study was to investigate gene differential expression in Pesudostellariae Radix from different provenances and habitats,as well as to isolate and evaluate associated differential genes.cDNA-AFLP was applied to analyze the differential expressed genes of Pesudostellariae Radix from two different provenances and habitats.Six primer pairs were selected,using these primer pairs,44 and 38 differentially expressed trivially distributed file system (TDFs),were amplified in Pesudostellariae Radix from same provenances and different habitats as well as same habitats and different provenances,respectively.These TDFs were cloned and sequenced,27 and 25 nucleotide sequences were obtained,respectively.By BLASTX analysis,19 and 16 of them were found with homologous sequences in databases,of which,7 and 6 TDFs had their known function,respectively.These proteins were mainly involved in the growth and development of plants,resisting diseases and insect pests,improving the ability of plants to resist the abiotic stress.Our research provided the basic information for revealing the molecular mechanism of Pesudostellariae Radix.

Key words: Pesudostellariae Radix, provenances and habitats, cDNA-AFLP, differential expressed genes