Abstract: In this study,the preventive effect of mangiferin (MGF) on dextran sulfate sodium (DSS) induced ulcerative colitis (UC) in mice and its influence on inflammatory cytokines were investigated.KM mice were randomly divided into blank control group,model control group,SASP positive control group (900 mg/kg),MGF groups (300,150,75 mg/kg) and MGF-SD group (equivalent to 300 mg/kg of MGF).Ulcerative colitis in mice model induced by DSS was prepared within 14 days.After intervened with medical drug daily on time,disease activity index (DAI),colon gross morphological damage index (CMDI) and colon histopathology index (CMDI) should be recorded as a judgment to evaluate the effect.The contents of IL-1β,IL-4,IL-6 and IL-10 were detected by ELISA.As shown in the result,there were significantly increased for CHPI,DAI,CMDI scores and the IL-1β,IL-6 levels (P<0.05) in model group,but obviously decreased for IL-4,IL-10 levels.Compared to model group,CHPI,DAI and CMDI of MGF were significantly decreased (P<0.05),the level of IL-1β in MGF and MGF-SD groups were significantly decreased (P<0.05),the level of IL-6 in MGF high dose group and MGF-SD group were significantly down regulated (P<0.05),but there was no significant difference between the two groups (P>0.05),the same to the levels of IL-4 and IL-10 in all drug intervention groups.It was indicated that MGF showed obvious preventive effect against DSS induced ulcerative colitis in mice.It may be related to the down-regulation of IL-1 and IL-6 in colon tissues,and further to regulate the balance of pro-inflammatory cytokines and anti-inflammatory factors.Increasing the solubility of MGF could not result in the enhancement of this preventive effect.

Key words: mangiferin, solid dispersion, ulcerative colitis, dextran sulfate sodium, inflammatory cytokines, mechanism