Abstract: In order to provide references for the quality evaluation and clinical medication of Psudostellaria heterophylla,the contents and monosaccharide compositions of polysaccharide from tissue-cultured P.heterophylla and its wild correspondence were investigated.The polysaccharides were extracted from P.heterophylla by a water-extraction and alcohol-precipitation method and the contents were determined by anthrone-sulfuric acid colorimetric method.The obtained polysaccharides were sequentially treated with 0.5 M HCl-methanol,trimethyl-silane derivatization and GC-MS analysis was carried out to study its monosaccharide compositions.The results showed that the contents of polysaccharide in tissue-cultured and wild P.heterophylla were 19.05±1.38% and 22.63±2.18%,respectively.GC-MS analysis revealed that polysaccharide in tissue-cultured and wild P.heterophylla were both consisted of glucose,fructose,mannose,galactose,galacturonic acid,arabinose and rhamnose,but the proportion of monosaccharides varied remarkably in the polysaccharides from different origins.

Key words: Psudostellaria heterophylla, polysaccharide, monosaccharide composition, GC-MS, origin