NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (4): 561-567. doi: 10.16333/j.1001-6880.2016.4.017

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Extraction and in vitro Antioxidant Activity of Anthocyanins Pigment from Vigna angularis (Willd.) Ohwi et Ohashi Hulls

LI Fang,RUAN Chang-qing*,TANG Kai-xin,LI Wei-wei,ZHANG Xue-lu,SUN Ji-tao   

  1. Food College,Heilongjiang Bayi Agricultural University,Daqing 163319,China
  • Online:2016-04-30 Published:2016-05-13

Abstract: In order to explore new anthocyanins resources,extraction process for anthocyanin pigments from Vigna angularis (Willd.) Ohwi et Ohashi hulls was developed and optimized by single factor and L9(34) orthogonal experiments.The optimal conditions for the extraction of anthocyanins from V.angularis hulls was 60% ethanol as extraction solvent,solid-liquid ratio of 1:20 (g:mL),temperature of 40 ℃ and pH 2.0.Under these extraction conditions,the highest extraction amount for anthocyanins was 5.912 mg/g with extraction yield of 19.1% and purity of 3.06%.V.angularis hulls crude extract showed a strong O2·-, ·OH and DPPH radical scavenging ability. V. hulls can be utilized as a new type of anthocyanins resource.

Key words: Vigna angularis (Willd.) Ohwi et Ohash hulls, anthocyanins, extraction, antioxidant activity

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