Abstract: The aim of this study was to observe the long-term toxicity of argininyl-fructoul-glucose(AFG) in mice.A total of 80 ICR mice,were randomly divided into 4 groups,20 mice in each group,half male and half female.Each group were lavaged with physiological saline,AFG 30 mg/kg,2 g/kg and 6 g/kg,once a day,last for 90 days,and all animals were daily monitored for abnormalities and mortality.After 90 days,in order to obtain the information on the progression of toxicological changes,mice were fasted for 12h and were subsequently sacrificed by exsanguination followed by a full,detailed gross necropsy.Embedded tissues were sectioned to approximately 5-6 μm in thickness,stained with hematoxylin and eosin(H&E) before they were examined under a microscope.After administration for 60 days,some mice in AFG 6 g/kg group appeared of eyes redness,slow action and other phenomena.Compared with the control group,no significant differences in weight change and food consumption were detected in AFG 30 mg/kg,2 g/kg and 6 g/kg group.The liver and spleen index of AFG 6 g/kg group decreased significantly(P<0.01, P<0.05) for serum chemistry analyses,Crea of AFG 30 mg/kg and 2 g/kg groups increased significantly(P<0.05),the ALT and AST of AFG 6 g/kg group increased(P<0.05),TG also rise obviously(P<0.01).Other blood biochemical indexes of sample groups had no significant difference with these of control group.The existing data showed that the 90 d in the use of AFG had no obviously toxicity in mice.

Key words: argininyl-fructoul-glucose, ICR mice, long-term toxicity