Abstract: A strain of endophytic fungus was isolated from the leaves of Trifolium repens L.,and identified as Aspergillus fumigatus. The fungus was cultured on rice media and its ETOAC extract showed inhibitory activity against the germination of cabbage seeds.Five anthraquinone derivatives were isolated from this active portion,and were elucidated as 1-O-methyl emodin (1),8-O-methyl emodin (2),asperfumin (3),monomethylsulochrin (4),and trypacidin (5) on the basis of spectroscopic methods.The bioactivity of 1-5 was evaluated by using calcium current model.The results showed that 1 cause a persistent increase in cytoplasmic calcium levels in A549 cells.

Key words: Trifolium repens L., endophytic fungi, Aspergillus fumigatus, 1-O-methyl emodin, calcium influx