Abstract: The bioactive protein was isolated from Ming mung bean by the precipitation with 25% and 75% of saturated ammonium sulfate,ion exchange chromatography on CM-52 and Gel-filtration chromatography on Sephadex G-75.The result showed that the purified protein exhibited antifungal activity against Fusarium oxysporum f.sp.vasinfectum and Rhizoctonia solani Kuha. It had inhibitory effect on Staphylococcus Aureus, but the antibacterial activity was relatively weaker compared with antifungal activity.The molecular weight of the purified protein was between 29.7 and 33.8 kDa by HPLC analysis.

Key words: Ming mung bean, bioactive protein, isolation and purification, antimicrobial property