NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (8): 1219-1222.doi: 10.16333/j.1001-6880.2016.8.008

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Isolation and Identification of Taxol-producing Endophytic Fungus N-15

XI Xiao-yuan,ZHAO Sheng-ying,ZHANG Peng*,SONG Fa-jun,LEI Shi-kang,NING Yi   

  1. College of Life Sciences,SouthCentral University for Nationalities,National Committee Key Laboratory for Biotechnology,Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,Wuhan 430074,China
  • Online:2016-08-30 Published:2017-01-17

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Abstract: In this study,a taxol-producing endophyitc fungus N-15 was isolated from the inner barks of Taxus chinensis var.mairei.The results of high performance liquid chromatography (HPLC) analysis showed that the extracts of N-15 produced same chromatographic peaks and retention time as authentic taxol,Baccatin III and 10-deacetylbaccatin III (10-DAB),suggesting that the fungus N-15 can produce taxol,Baccatin III and 10-DAB.The results of mass spectrometry (MS) analysis showed that the fungal extracts of N-15 had the same mass peaks as authentic taxol,which further indicated that N-15 can produce taxol.The fungus N-15 was preliminarily identified to belong to Nigrospora sp. based on its morphology and conserved sequence of 18S rRNA.

Key words: Taxus, endophytes;taxol;baccatin III;Nigrospora sp.

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