Enzymatic Preparation of Karenia Polysaccharides and Its Anti-angiogenic Activity

doi:10.16333/j.1001-6880.2016.9.023

Abstract

Abstract: To investigate the preparation method on karenia polysaccharides with alkali protease enzymolysis from Karenia mikimotoi and its anti-angiogenic activity.The extraction of karenia polysaccharides was obtained by flash extraction combined with enzymolysis method,and the optimal process conditions were determined as enzymolysis temperature of 40 ℃,pH 8.5,enzymolysis dosage of 2.5% and enzymolysis time of 2 h.The karenia polysaccharides were obtained by using trichloroacetic acid to get rid of protein and ethanol for precipitation.The karenia polysaccharides showed some inhibition on the viability and migration of induced human umbilical vein endothelial cells at certain concentration.

Key words: Karenia mikimotoi, polysaccharides, flash extraction enzymolysis, anti-angiogenic activity

CLC Number:

O629.1

CHEN Yu-ting1,MENG Fan-tong1,2,KONG Liang1,TIAN Xi-fan1,TAN Cheng-yu1*. Enzymatic Preparation of Karenia Polysaccharides and Its Anti-angiogenic Activity[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(9): 1470-1474.

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Enzymatic Preparation of Karenia Polysaccharides and Its Anti-angiogenic Activity

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