Abstract: To establish an HPLC method for simultaneous determination of catalpol,rehmannioside D,leonuride,acteoside,isoacteoside in Rehmanniae Radix.Synergi^TM Hydro-RP 80 column (250 mm× 4.6 mm,4 μm)was used,with acetonitrile-0.2% phosphoric acid as the mobile phase in gradient elution mode.The column temperature was 35 ℃,with flow rate1.0 mL/min.The UV detection wavelength was set at the maximum absorption wavelength,203 nm for catalpol,rehmannioside D and leonuride,334 nm for acteoside and isoacteoside.The five kinds of glycosides in Rehmanniae Radix were separated well,and the linear relation was obtained(r≥0.9990).The average recoveries were 96.43%,97.52%,96.16%,100.48%and 97.21%,with RSD 1.44%, 2.56%,1.07%,1.98%,3.35%,respectively.Themethod is simple,accurate,reproductive,and can provide the basis for the quality control of Rehmanniae Radix.

Key words: Rehmanniae Radix, HPLC, catalpol, rehmannioside D, leonuride, acteoside, isoacteoside