Abstract: Antifreeze proteins (AFPs) obtain more and more extensive concerns recently because of their unique structures and functions.In the research of antifreeze proteins,the evaluation of antifreeze activity is the first step and the most critical step.The accuracy and comparability of the measurable results directly affects its followup studies.In this article,the advantages and disadvantages of existing antifreeze activity evaluation methods are reviewed.The relationship,present problems and development trend,among different evaluation methods of the antifreeze activity,are analyzed and compared.The standardization,accuracy and reproducibility of evaluation methods of the antifreeze activity is the emphasis of the future development.

Key words: antifreeze protein, antifreeze activity, evaluation methods, progress