Abstract: The separation and purification of vaccarin in Semen Vaccariaewith macroporous resin had been studied in this research.The refinement process and adsorption factor were compared by static and dynamic adsorbing experiments.Vaccarin was developed as its quality standard,screening out the best refinement process and adsorption factor.The optimized refinement process and adsorption factor were stable and feasible.The effect of vaccarin on the proliferation of endothelial cells was detected by SRB assay.The result of this studyprovidedreference for the refinement of Semen Vaccariaewith macroporous resin.In addition,it was also found that vaccarin markedly promoted the proliferation of endothelial cells.

Key words: Semen Vaccariae, vaccarin, macropotous resin, static adsorption, dynamic adsorption, cell proliferation