Abstract: The cross-linked starch microsphere(PCSM) was synthesized from potato starch by W/O emulsion method at room temperature,while its surface charge was modified with 2-dimethylaminoethyl.Meanwhile,the preparation and modification processes were studied,and the suitable starch microcarrier was successfully made for cell cultivation.The microspheres were characterized by scanning electron microscopy(SEM),Fourier translation infrared spectrum(FTIR) and X-ray diffraction(XRD).The results showed that the optimal parameters for the preparation were as follows:the starch solution concentration of 7%,the mixing speed of 500 RPM,the crosslinking agent dosage of 8%,and the oil/water volume proportion of 1:6.The optimal parameters for the modification process were found:2 times the mass of microspheres in volume of 3.5 mol/L NaOH solution and 2.5 mol/L DEAE-HCl solution added,and then stirred at 60 ℃ for 4 h.Compared with Cytodex-1,after culturing CHO-K1 for 144 h,the cell density on the surface of starch microcarrier reached 1.8 x 10⁶ cells/mL.The culturing effect remained similar for both microcarrier,suggesting that the homemade starch microcarrier is a kind of potential high polymer material for adherent cell culture.

Key words: potato starch, starch microspheres, 2-dimethylaminoethyl, CHO-K1 cells, cell culture