Abstract: In order to improve the production efficiency of Armillaria mellea, taking Trifolium repens L.as the main material of medium,the optimization of the culturing conditions was carried out using orthogonal analytical method after single factors screening,including the consumption of T.repens and glucose,as well as the boiling time.The optimal formula was the boiled mixture of T.repens 100 g/L,glucose 20 g/L and agar 20 g/L.Using this new medium,the germination time of A.mellea was decreased from 6 d in the PDA to 2 d,while the dry weight of mycelium reached to 0.0216 g/d,2.8 times of that in PDA.

Key words: Armillaria mellea, Trifolium repens L., modified medium, formulation optimization