Abstract: To understand the chemical constituents of Pseudostellaria heterophylla for clarifying its pharmacological basis,different types of column chromatography (CC) including macroporous resin CC,silica gel CC,reversed phase C18 CC and Sephadex LH-20 CC were employed for their isolation and purification.As a result,ten compounds were isolated and structurally determined as 3-furanmethanol α-D-galactopyranoside (1),piceid (2),dihydroferulic acid methyl ester (3),benzoic acid (4),caffeine (5),thymidine (6),salicylic acid (7),dihydroferulic acid (8),adenosine (9) and heterophyllin B(10) on the basis of NMR and HRMS.1 was a new compound,and 2-7 were isolated from this plant for the first time.

Key words: Pseudostellaria heterophylla, chemical constituents, 3-furanmethanol &alpha, -D-galactopyranoside