Screening Methods of Trace Alkaloids Components from Semen Zizyphi spinosae

doi:10.16333/j.1001-6880.2017.7.015

Abstract

Abstract: To accurately and quickly screen and identify trace alkaloids components from Semen Zizyphi spinosae,a HPLC online separation method of alkaloids components was developed using the columns which belong to the nitrogen-containing organic compounds (alkaloids) analysis kit.Then the alkaloids components separated on line were identified with the help of MassWorks molecular recognition software and LC-ESI-MS/MS.Three compounds:sanjoinine K (C₁₇H₁₉NO₃),caaverine (C₁₇H₁₇NO₂) and Nmethylasimilobine (C₁₈H₁₉NO₂),belong to aporphine alkaloids and two compounds:sanjoinine F (C₃₁H₄₂N₄O₅) and sanjoinine A (C₃₁H₄₂N₄O₄),belong to cyclopeptide alkaloids were screened and identified.In addition,there was a pair of isomeric compounds:magnoflorine and zizyphusine (C₂₀H₂₄NO₄),which belong to aporphine alkaloids to be further identified.A rapid and efficient screening method for alkaloids from Chinese medicine was established in this study.

Key words: Semen Zizyphi spinosae, alkaloid, MassWorks, LC-ESI-MS/MS

HU Yan-zhou,DING Ke*,HAN Tao,CHEN Xiang-ning. Screening Methods of Trace Alkaloids Components from Semen Zizyphi spinosae[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(7): 1165-1170.

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Screening Methods of Trace Alkaloids Components from Semen Zizyphi spinosae

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