Abstract: The aim of this study was to explore the improvement function of Shizidaiping on insulin resistance of type 2 diabetic SD rats model and the influence on the expression of musculi skeleti insulin signal transduction proteins PI3K,P-PI3K and GLUT4 as well as to provide experimental basis for the clinical application of Shizidaiping.Breeding induced SD rats with type 2 diabetes insulin resistance model was developed using streptozotocin,high fat and sugar.Practice lavage intervention on model rats with high,medium and low doses of Shizidaiping and set up normal control group,model group and metformin group.Eight weeks later,practice normal saline lavage for normal group and model group.Fasting blood glucose and glucose tolerance test were carried out before using the medicine,after 4 and 8 weeks of using medicine.After 8 weeks of using the medicine,glycosylated hemoglobin (GHB),glycosylated serum protein (gsp) and c-peptide (c-p) were examined.Insulin resistance index and protein expression level of musculi skelet were calculated.Then statistical analysis was carried out using SPSS 20.0 software.Compared the treatment groups with the model groups after 8 weeks of using the medicine,it was found that FBG,PG2h and AUC decreased significantly (P<0.05 or P<0.01).After the treatment finished,there was obvious difference between the treatment groups and the model groups on GHB,GSP,C-P and HOMA-IR (P<0.05 or P<0.01).There was no obvious difference between different groups on musculi skeleti PI3K (P>0.05).The protein expression level of P-PI3K and GLUT4 of the treatment groups was significantly higher than these of model groups (P<0.05).Therefore,Shizidaiping can reduce blood glucose of type 2 diabetic rats and improve insulin resistance (IR).Its pharmacological mechanism was possibly through improving of protein expression level of P-PI3K and GLUT4 of musculi skeleti.

Key words: Shizidaiping, glycometabolism, musculi skeleti insulin resistance, PI3K/GLUT4