Abstract: Liver fibrosis is the pathological repair response of liver to injury and characteristic of activation and expanding of hepatic stellate cells (HSC) and excessive accumulation of extracelluar matrix proteins (ECM) in liver.CCN factors are the signaling matricellular protein,involving in tissue repair and fibrogenesis.In this study,the highly pure primary rat HSC was isolated and cultured for their transition into the myofibroblastic phenotype.These cultured HSCs were daily collected and characterized for the MF phenotypic features,and then mRNA expression of all CCNs genes within these transiting HSCs were analyzed with the real time QRT-PCR for a full assessment of the roles of CCNs during HSC trans-differentiation.

Key words: CCN family, hepatic stellate cell, hepatic fibrosis