Abstract: To observe the effect of β-Asarone and tenuigenin on the regulation of SOD, GSH-PX, CAT, MDA and HO-1 in APP/PS1 double transgenic mice, to explore the protection mechanism of β-Asarone and tenuigenin for oxidative stress injury of double transgenic mice and treatment of AD. Three-month-old of APP/PS1 double transgenic mice were divided into model group,Donepezil hydrochloride group (0.33 mg/kg·d), high dose of β-Asarone and tenuigenin group, medium dose of β-Asarone and tenuigenin group and low dose of β-Asarone and tenuigenin group (18.5,37 and 74 mg/kg·d). C57BL/6 mice of the same age were used as control. Morris water maze test and object recognition task were used to measure the spatial learning and memory ability. Spectrophotometer was used to detect the activities of SOD, CAT, GSH-PX and the content of MDA in mice. Western Blot and RT-PCR were adopted to observe the levels of mRNA and protein expression of CAT and HO-1 in each group of mice. The results showed that β-Asarone and tenuigenin treatment significantly ameliorated the cognitive deficits of APP/PS1 double transgenic mice. β-Asarone and tenuigenin increased the activity of SOD,CAT and GSH-PX,decreased the production of MDA,enhanced the mRNA and protein expression of CAT and HO-1.Hence, β-asarone and tenuigenin can inhibit the oxidative stress of brain tissue,and bring positive therapeutic effect of AD by regulating the activity of SOD,GSH-PX,CAT,MDA and HO-1.

Key words: β-asarone, tenuigenin, SOD, GSH-PX, CAT, MDA, HO-1