Abstract: To study the effects of bergapten (5-MOP) on proliferation and differentiation of cultured osteoblasts in vitro,MTT,microtitration assay,enzyme-linked immunosorbent method (ELISA) and qPCR were used separately to observe the proliferation,the activity of ALP and the content of osteocalcin and mRNA expression of collagen I of osteoblasts cultured in vitro with bergapten (5-MOP) of different concentrations in it for different incubation periods.The effect of promoting proliferation of rat osteoblasts cells in vitro after 24 h and 48 h and 72 h was more marked by the groups of bergapten (5-MOP) compared with the control group.The activity of alkaline phosphate (ALP) and secretion of osteocalcin (BGP) and mRNA expression of collagen I were stimulated by bergapten (5-MOP) after 48 h and 72 h incubation.It was demonstrated that bergapten (5-MOP) had the effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro.

Key words: osteoblast, proliferation, differentiation, bergapten