Abstract: The purification process of atractylenolide I and atractylenolideII from Codonopsispilosula were studied.Adsorption capacity,desorption capacity,adsorption rate and desorption rate were selected as evaluation indexes,the types of macroporous resin was screened and the purification parameters were further optimized.The optimal purification conditions were as follows:DM130macroporous resin was chosen,30 ℃was the optimal temperature for adsorption,the concentration of sample solution was 0.125 mg/mL,the volume of sample solution was 200 mL,adsorption flow rate was 6 mL/min,and eluted with 95% ethanol,elution flow rate was 4 mL/min.Under these conditions,atractylenolide I and atractylenolide II showed 32.3,and 25.6 fold increases in solid content,respectively.

Key words: Codonopsispilosula, atractylenolide I, atractylenolide II, macroporous resin