Determination of Molecular Weight Distribution of Eucheuma muricatum Polysaccharide by HPLC-CAD

doi:10.16333/j.1001-6880.2017.S.022

Abstract

Abstract: The Molecular weight distribution of Eucheuma muricatum polysaccharide was measured by using High Performance Liquid Chromatography combined with CAD detector and gelpermeation chromatograph,dextran was used as the standard solution and mapping analyzed by Chromeleon 7 Extension Pack GPC Templates software.The average molecular weight of Eucheuma muricatum polysaccharide is 356657 and the weight average molecular weight is 721611,which is mainly distributed between 180000 and 820000.Polydispersity PD is 2.02,The method is simple,rapid and accurate.

Key words: Eucheuma muricatum polysaccharide, High Performance Liquid Chromatography, molecular weight distribution, molecular weight

LIN Shun-quan1,HUANG Jing1,LI Jin-ju1,YUAN Xiao1,2*. Determination of Molecular Weight Distribution of Eucheuma muricatum Polysaccharide by HPLC-CAD[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(增刊1): 103-105.

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Determination of Molecular Weight Distribution of Eucheuma muricatum Polysaccharide by HPLC-CAD

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